Biogenic Synthesis of Silver Nanoparticles Using Aqueous Solution of Cherimoya Seeds Extract and Its Antimicrobial Activity

In this study, we report a simple and eco friendly method for synthesis of well dispersed and stable silver nanoparticles (AgNPs) using cherimoya seeds extract as both reducing and stabilizing agents in aqueous solution without employing any other capping and reducing agents. The arrangement of silver nanoparticles was observed by change of color from pale yellow to dark brown and the UV– vis absorption spectroscopy. The outcome of reaction time and cherimoya seeds amounts on the synthesis of silver nanoparticles was studied. The Size, morphology and composition of nano particles (NPs) were studied by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) which showed that the silver nanoparticles were agglomerated spherical in shape with a size distribution  85 nm. The crystalline structure of the silver nanoparticles was recognized by X-ray diffraction technique (XRD). The information of elemental compositions for AgNPs was carried by Energy dispersive X-ray spectroscopy (EDX). The synthesized silver nanoparticles were tested against bacteria (Gram positive such as Staphylococcus aureus and Bacillus subtilis and Gram negative such as Escherichia coli and Klebsiella pneumoniae) and fungi (Aspergillus niger and Candida albicans) and they obtained data were indicative of good antibacterial properties of the materials. The method obtainable in this paper provides a very promise move toward to synthesize other noble nanoparticles using renewable materials as capping and reducing agents.